Epigenetic Regulation of Stem cells and Cancer

Epigenetic Regulation of Stem cells and Cancer2020-05-13T13:27:25+02:00

Project Description

Manching Ku, PhD

E: manching.ku@uniklinik-freiburg.de
T: +49 (0)761 270-44000

Department of Pediatric Hematology and Oncology
Center for Pediatrics and Adolescent Medicine
Mathildenstraße 1
79106 Freiburg
Germany

We are interested in understanding the epigenetic and transcriptional regulation of cell state transition in cancer and immunology. We employ next-generation sequencing methods to investigate genetic and epigenetic aspects of cancer pathogenesis, metastasis and cancer immunotherapy.

TEAM

Dr. Sarina Halm

Darius Halm

Natascha Roser

Kerstin Bruder

Jessica Heinze

CURRENT PROJECTS

Chromatin regulation of embryonic stem cells

We aim to understand chromatin dynamics during cell state transition in the context of regenerative medicine. Using multi-omics techniques, such as Chromatin-immunoprecipitation coupled with next generation sequencing (ChIP-Seq), we interrogate the roles of polycomb proteins and histone variants in various stem cells and cancer cells.

Heterogeneity of regulatory T cells

Using single cell RNA-Seq techniques, we have discovered heterogeneity in regulatory T cells. Each Treg subset represents differential transcriptional signatures. We continue to explore and identify additional subsets to understand their functions.

Assay Development for Clinical Cancer Diagnostics

We develop clinical diagnostic next-generation sequencing methodologies to investigate gene mutations and epigenomes in pediatric cancers.

SELECTED RECENT PUBLICATIONS

Google Scholar: https://scholar.google.com/citations?user=x5bquwoAAAAJ&hl=en

Pubmed: https://www.ncbi.nlm.nih.gov/pubmed/?term=manching+ku

*equal contribution, ^corresponding author.

COLLABORATIONS

Dr. med. Oktay Kirak, Freiburg Center for Pediatrics and Adolescent Medicine

Prof. Melanie Boerries, University of Freiburg

PD Dr. med. Simone Hettmer, Freiburg Center for Pediatrics and Adolescent Medicine

Prof. Tak Sing Wong, Pennsylvania State University

Prof. Kathy Lui, Chinese University of Hong Kong

Dr. Jerome Mertens, University Innsbruck

FUNDING

DFG SFB1160 IMPATH

DAAD German Academic Exchange Service

University of Freiburg / Penn State University Global Programs

Qiagen NGS Sponsorship Award